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KMID : 0380020040190060441
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 6 p.441 ~ p.445
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Cellular Iron Uptake from Aqueous Solutions depending on Reaction Conditions by genetically engineered Saccharomyces cerevisiae
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Kim Sang-Jun
Chang Yu-Jung
Park Chung-Woong
Jeong Yong-Seob
Kim Kyung-Suk
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Abstract
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Cellular iron uptake was performed in the yeast Saccharomyces cerevisiae that transformed with human ferritin H- and L-chain genes. The recombinant yeasts were enriched in YEP medium supplemented with galactose for 3 days and the iron uptake was followed by incubating the cells with iron in 20 mM MOPS buffer (pH 6.5). The reactions were examined under different conditions including the iron compounds of Fe(II) and Fe(III), the concentration of iron, the concentration of cells and the reaction time. From our results, the recombinant yeast YGH2 producing H-chain ferritin showed higher cellular iron concentration at the cell concentration of 100 mg/ml than 200 mg/ml. Iron presented as Fe(II) rather than Fe(III) was taken up more efficiently. Iron uptake increased slightly when iron was added up to 14.3 mM Fe(II) and then its cellular iron concentration was cell wet wt. In addition, the iron uptake reaction reached to maximum at about 2 hr incubation.
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KEYWORD
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Cellular iron uptake, recombinant yeast, ferritin gene
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